May 11 2011 03:57 PMLaboratory Products
Quick and reliable determination of complete proteins in pharmaceutical products by way of a TOC/TNb Analyser
In the pharmaceutical industry, the protein contents in culture media (for example, in vaccines) often have to be determined within the context of quality controls of raw materials, intermediate and finished products. Today a broad range of methods is available for this purpose. The European Pharmacopeia (Ph.Eur. 2.5.33) alone describes seven different methods that are based on different reaction and detection principles. The largest group of such methods consists in protein tests according to Lowry, Bradford, BCA, and Biuret which are all based on different colouring reactions with certain amino acid rests, followed by an absorption measurement at certain wavelengths in the visible spectral range. In addition, the pharmacopeia contains descriptions of the direct UV absorption method, a fluorescence analytical method as well as two methods based on the nitrogen content measurement.
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